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Tobias Lammel

Postdoktor

Tobias Lammel
Postdoktor
031-786 3625
0766-183625

Postadress: Box 463, 40530 Göteborg
Besöksadress: Medicinaregatan 18 , 41390 Göteborg


Institutionen för biologi och miljövetenskap (Mer information)
Box 463
405 30 Göteborg
www.bioenv.gu.se
bioenv@bioenv.gu.se
Besöksadress: Medicinaregatan 18 , 413 90 Göteborg

Om Tobias Lammel

At present I am involved in the following projects.  If you would like to know more and/or are interested in working with us (for example in form of a Bachelor/Master/doctoral thesis or a more formal collaboration) please do not hesistate to contact me (tobias.lammel@gu.se).

 

Implementation of novel tools to assess metal nanoparticle uptake and trophic transfer (Acronym: NanoTransfer)

Implementation of novel tools to assess metal nanoparticle uptake and trophic transfer(Acronym: NanoTransfer)Summary: While uptake and effects of engineered metal nanoparticles (metal ENPs) in short-term water-only laboratory tests have been widely examined in controlled freshwater systems, only a few studies have focused on dietary/sediment exposure or long-term effects. Even less emphasis have been directed to understanding mechanisms controlling uptake and internal fate and only a very few studies exist on trophic transfer of metal ENPs. Advancement has been restrained due to limitations regarding available characterization methods (both in biological and environmental samples). The project will combine and use a toolbox of novel and advanced techniques in microscopy, and ENP labelling (stable isotopes and fluorescence) in combination with Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and single-particle ICP-MS (SP-ICP-MS) to address these knowledge gaps. We intend to take advantage of these new tools to examine trophic transfer of metal ENPs and address how exposure time affects uptake, internal fate and toxicity of metal ENP in prey (sediment-dwelling worm) and predator (fish).

 

Mixture toxicity of Engineered NAnomaterials and Chemicals in the Environment (Acronym: MENACE)

Engineered nanomaterials (ENMs) represent a novel class of contaminants in aquatic ecosystems. There is increasing concern that their co-existence and interaction with “traditional”, i.e. chemical environmental pollutants may enhance their respective hazards to aquatic biota. ENMs are able to cross biological barriers that are typically insurmountable for larger particles and can thus, taking into account their high specific surface area and adsorption ability, act as vector for surface-bound toxic chemicals (“Trojan horse”-effect). In addition, ENMs and chemical pollutants may elicit toxicity on similar endpoints leading to enhanced toxicity via additive or synergistic effects. The objective of this project is to elucidate if and to which extent the above processes occur in and represent a threat to fish (proof of concept). Specifically, we will investigate if co-exposure to specific ENMs and chemical environmental pollutants, e.g. titanium dioxide (TiO2) nanoparticles (NP) and polychlorinated biphenyls (PCB), can disrupt the integrity of the piscine gut epithelium and thereby facilitate each other’s uptake into the animal. It aims at casually linking effects of ENM/chemical-mixtures on the molecular/cellular level with adverse effects at the tissue and organism level (phenotypic anchorage) combining state-of-the-art transcriptomics, an advanced in vitro model system mimicking the piscine gut epithelium, and in vivo biomarker and bioaccumulation studies.


Development of three-dimensional (3D) spheroidal aggregate cultures from the continuous rainbow trout liver cell line RTL-W1

At present primary hepatocytes are the preferred model for mimicking liver-typic functions in vitro, in particular for studies investigating xenobiotic biotransformation and clearance. However, primary hepatocytes have certain shortcomings: Rapid loss of metabolic competencies and limited longevity make them unsuitable for long-term toxicity studies; isolation of cells from the animal and subsequent functional characterization/quality control is time- and labor intensive, use of cells originating from fish with different genetic and biochemical background causes inter-study variability, and last but not least, isolation of cells implies killing of fish giving rise to ethical concerns. Continuous liver cell lines are superior to primary hepatocytes regarding most of the above aspects, but their capability to reproduce complex toxic responses as they would occur in vivo is limited, due to partial or complete loss of certain tissue-specific functionalities at time of their establishment. It has been demonstrated that culturing cells in a more natural, three-dimensional (3D) environment allowing for cell-cell interaction and communication assists restoring tissue-specific cell shape, polarity, and functionalities. The objective of this project is to develop and functionally characterize 3D spheroidal aggregate cultures from the rainbow trout liver cell line RTL-W1 and investigate their potential for environmental toxicity testing of chemicals and nanomaterials.

 

 

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2019

Development of three-dimensional (3D) spheroid cultures of the continuous rainbow trout liver cell line RTL-W1
Tobias Lammel, Johanna Jellinek, Joachim Sturve
Ecotoxicology and Environmental Safety, Artikel i vetenskaplig tidskrift 2019
Artikel i vetenskaplig tidskrift

2018

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Sidansvarig: Sven Toresson|Sidan uppdaterades: 2015-06-29
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